U-Idaho Biologist Investigates a Common Virus’ Damaging Effect on Cells

Thursday, November 29 2012

MOSCOW, Idaho - University of Idaho biologist Lee Fortunato’s innovative new research method reveals how a virus responsible for birth defects co-opts a cell’s ability to repair itself.

Fortunato, an associate professor of biological sciences, has spent more than 15 years studying human cytomegalovirus (HCMV), and her recent research was published today in the Public Library of Science’s “Pathogens” journal. Though HCMV is common – 50-80 percent of humans are carriers – it can cause severe birth defects if a fetus is infected in utero.

Approximately 1 percent of babies born annually in the United States – about 40,000 – are infected with HCMV. Among them, some 4,000 are born with such conditions as loss of vision and hearing, cerebral palsy, mental retardation and microcephaly, or small head size. Another 4,000 develop problems, such as progressive hearing loss, during childhood.

Fortunato’s work examines the effects of the virus on cellular DNA repair mechanisms. It could lead to an antiviral therapy and new understanding of the ways infections caused by various disease-causing organisms defeat cells’ defenses.

When HCMV infects a cell, it sets up centers inside the nucleus to replicate itself. The proteins that repair cellular damage – including one known as the “guardian of the genome” – become sequestered inside these centers.

Fortunato hypothesized the virus was taking over the cell’s repair mechanisms and using them for itself, leaving the cells unable to fix themselves if they incurred damage or kill themselves to stop the spread of the virus.

She exposed infected cells to UV irradiation, which equally damaged the cellular and viral DNA. She then recorded the rate of repair using an established assay.

Unlike typical studies that don't differentiate between cellular and viral DNA in the infected cells, Fortunato's test examined repair in both genomes separately, yet simultaneously. The test showed the cellular machinery repaired the viral DNA far more quickly and efficiently than the cellular DNA.

“We’ve shown that, at least with UV irradiation of an infected cell, the viral genome is preferentially repaired, but the cellular genome is not repaired,” Fortunato said. “That could have ramifications for an infected fetus.”

Fortunato said it is very likely the virus keeps cells from repairing themselves when damaged by factors other than UV radiation. Other researchers could use her method for comparing DNA damage to prove it.

Following Fortunato’s method for testing cellular DNA separately from invasive DNA could open doors in other research as well. And, in the long term, Fortunato’s work could greatly reduce the negative ramifications of HCMV infections.

View the full paper at the www.plospathogens.org/article/info%3Adoi%2F10.1371%2Fjournal.ppat.1003038.

# # #

About the University of Idaho
The University of Idaho inspires students to succeed and become leaders. Its land-grant mission furthers innovative scholarly and creative research to grow Idaho's economy and serve a statewide community. From its main campus in Moscow, Idaho, to 70 research and academic locations statewide, U-Idaho emphasizes real-world application as part of its student experience. U-Idaho combines the strength of a large university with the intimacy of small learning communities. It is home to the Vandals, and competes in the Western Athletic Conference. Through the university’s $225 million Inspiring Futures capital campaign, private giving will enhance student learning, faculty research and innovation, and a spirit of enterprise. Learn more: www.uidaho.edu.