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Integrated Quantitative Phase and Airy beam Light Sheet Imaging

OTT Case 19-020 | Patent Pending

The invention combines Quantitative Phase Imaging (QPI) and Airy beam Light Sheet Imaging (LSI) onto a standard inverted microscope. This microscope design integrates the two modalities without any disruptions in their respective optical paths. Preliminary test results indicate a compatibility of the integrative design with microfluidics in a representative investigation of the effects of cellular noise on metabolic compartmentalization. The use of microfluidics critically alleviates some of the stringent culture and sample preparation techniques required by most LSI and Lattice LSI configurations. The design is inherently compatible with most QPI methods, such as holographic tomography.