IVIS Imaging Service Center
The IVIS Imaging Service Center provides access to fluorescence and luminescence imaging technology through supervised use of the IVIS Lumina III. The Lumina III can be used for imaging of living prokaryotes (e.g., bacterial plates, films), invertebrates (e.g., nematodes, insects), fungi, plants and vertebrates (e.g., fish, mammals, birds) and ex vivo tissue specimens. Some examples from the published literature are provided below.
Have a question or want to schedule a training to use the Lumina III?
If you are interested in using the IVIS Imaging Service Center, you will need to complete the online training and schedule in-person training. Once training is completed, we can schedule your approved experimental protocol that is consistent with IVIS Lumina III capabilities and image analysis. To inquire about training and using the IVIS Lumina III, please contact Shirley Luckhart, Institute for Health in the Human Ecosystem, and Kiah Stewart, U of I Lab Animal Research Facility.
Technology Access Grants (TAGs) available
Applications are now open for IHHE IVIS Imaging Service Center Technology Access Grants (TAG). IHHE will fund up to five (5) TAGs for U of I investigators interested in using this facility for experimental research with fluorescence and bioluminescence imaging technology, through supervised use of the IVIS Lumina III. TAG support for up to $1,200 per project will be awarded for hours of training provided by the service center, for use of the IVIS Lumina III and for data analysis completed on service center computers. U of I investigators, postdoctoral fellows, graduate students and other research group members are eligible to apply.
Training materials
- In vitro Bioluminescence videos | Part One | Part Two |
- In vitro Fluorescence videos | Part One | Part Two |
- Advanced imaging of fluorescent and bioluminescent probes (pptx)
- Software manual (pdf)
How can I use the IVIS?
- Bioluminescence imaging of c-fos gene expression accompanying filial imprinting in the newly hatched chick brain (pdf)
- Filial responses as predisposed and learned preferences: Early attachment in chicks and babies (pdf)
- Critical role of the neural pathway from the intermediate medial mesopallium to the intermediate hyperpallium apicale in filial imprinting of domestic chicks (Gallus gallus domesticus) (pdf)
- Development of a chinchilla model to allow direct, continuous, biophotonic imaging of bioluminescent nontypeable Haemophilus influenzae during experimental otitis media (pdf)
- A mutation in the sap operon attenuates survival of nontypeable Haemophilus influenzae in a chinchilla model of otitis media (pdf)
- Bioluminescence imaging of live infected salmonids reveals that the fin bases are the major portal of entry for Novirhabdovirus (pdf)
- The major portal of entry of koi herpesvirus in Cyprinus carpio is the skin (pdf)
- In vivo monitoring of Yersinia ruckeri in fish tissues: progression and virulence gene expression (pdf)
- An ultrasensitive NanoLuc‑based luminescence system for monitoring Plasmodium berghei throughout its life cycle (pdf)
- Bioluminescent imaging of Trypanosoma cruzi infection in Rhodnius prolixus (pdf)
- CpxRA contributes to Xenorhabdus nematophila virulence through regulation of lrhA and modulation of insect immunity (pdf)
- In vivo imaging of Chikungunya virus in mice and Aedes mosquitoes using a Renilla Luciferase clone (pdf)
- Molecular pathogenesis of Listeria monocytogenes in the alternative model host Galleria mellonella (pdf)
- Monitoring of the parasite load in the digestive tract of Rhodnius prolixus by combined qPCR analysis and imaging techniques provides new insights into the trypanosome life cycle (pdf)
- Paratransgenesis: An approach to improve colony health and molecular insight in honey bees (Apis mellifera)? (pdf)
- Visualization and live imaging analysis of a mosquito saliva protein in host animal skin using a transgenic mosquito with a secreted luciferase reporter system (pdf)
- Molecular pathogenesis of Listeria monocytogenes in the alternative model host Galleria mellonella (pdf)
- Fetal gene transfer using lentiviral vectors: In vivo detection of gene expression by microPET and optical imaging in fetal and infant monkeys
- Long-term luciferase expression monitored by bioluminescence imaging after adeno-associated virus-mediated fetal gene delivery in Rhesus monkeys (Macaca mulatta) (pdf)
- Real-time bioluminescence imaging of macroencapsulated fibroblasts reveals allograft protection in Rhesus monkeys (Macaca mulatta) (pdf)
- Bioluminescence imaging of Clavibacter michiganensis subsp. michiganensis infection of tomato seeds and plants (pdf)
- Combined treatments reduce chilling injury and maintain fruit quality in avocado fruit during cold quarantine (pdf)
- Determination of rachis color loss by IVIS fluorescence in table grapes
- Leveraging management strategies for seedborne plant diseases to reduce Salmonella enterica Serovar Typhimurium incidence on tomato seed and seedlings (pdf)
- Using spontaneous photon emission to image lipid oxidation patterns in plant tissues (pdf)
- Visualization of plant viral suppressor silencing activity in intact leaf lamina by quantitative fluorescent imaging (pdf)
- Increased anthocyanin and flavonoids in mango fruit peel are associated with cold and pathogen resistance (pdf)
Rats
- Maltodextrin-based imaging probes detect bacteria in vivo with high sensitivity and specificity
- Low-amplitude ultrasound enhances hydrodynamic-based gene delivery to rat kidney
- Whole animal in vivo imaging after transient, nonviral gene delivery to the rat central nervous system
- Multimodality imaging for vertebral metastases in a rat osteolytic model
- Nanoparticles effectively target rapamycin delivery to sites of experimental aortic aneurysm in rats
- In vivo tracking of human neural progenitor cells in the rat brain using bioluminescence imaging
- Pulse probiotic administration induces repeated small intestinal Muc3 expression in rats
- Development and validation of a multi-dose neutropenic rat thigh infection model using real-time monitoring of Staphylococcus aureus growth in vivo (pdf)
- In vivo imaging of pathogen homing to the host tissues (pdf)
Mice
- Development of a red fluorescent labeled agent for assessing HER2 expression (pdf)
- Development of a near infrared fluorescent labeled agent for assessing bombesin receptor expression in cancer (pdf)
- Stem cell research and regenerative medicine (pdf)
- Cerenkov imaging of radioisotopes in IVIS systems (pdf)