| We are pleased to announce services available through the UI Automated DNA Sequencing Facility. For a nominal fee, we will process user supplied samples for sequencing. |
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Automated DNA sequencing on the Licor 4000L has some advantages over the more common ABI instruments. The primary advantage is read length. The Licor 4000L can routinely provide 800-1000 bases of reliable sequence data at greater than 98.5% accuracy. The key to automated sequencing is quality of the DNA template. That is why we will also purify plasmid, if so desired. For those who prefer to prepare their own plasmid preparations, we will provide information and protocols on how to purify and quantitate plasmid preparations for automated DNA sequencing. |
| Most common sequencing/cloning vectors have primer sequences necessary for cycle
sequencing using labeled primers. Currently we stock M13Forward, M13Reverse, T3 and T7
primers. If there is a demand, we will obtain SP6, KS, SK or other primers. Those who wish
to directly sequence PCR products, these need to be generated with a primer sequence as a
5' extension. They also require some purification in order to obtain long read lengths.
Contact us for more information on directly sequencing PCR products. Data can be provided
to clients by a number of methods. Among these, we can simply email to clients sequence as
GCG compatible text files, or provide SCF files. Depending on demand on the instrument, turn around time is usually about 10 working days. |
For additional information please contact:
Dr. Pat Shiel (pshiel@uidaho.edu)
Phone: 885-5867
Department of Plant, Soil and Entomological Sciences
AgSci Bldg. Rm 242
University of Idaho
Moscow, Idaho 83844-2339